Episode Details

With the prevalence of Leaf Roll Three, Red Blotch, and other viruses, accurate and timely detection of viruses in grapevines has never been more imperative. Alan Wei, Owner and Lab Manager at Agri-Analysis LLC in Davis California explains how his lab is using state-of-the-art technologies to find new viruses. Dr. We will explain sampling strategies to help growers screen and test larger number of grapevines with a fixed testing budget. He will also explain exciting emerging technologies such as next generation sequencing (NGS). Currently, polymerase chain reaction (PCR) is the widely accepted method for testing for viruses. This process tests for one gene at time. Next generation sequencing allows labs to test multiple genes at a time and potentially discover unknown viruses from a single test.

• 20: Dr. Mark Fuchs | Red Blotch Virus in Grapevines
• 49: Stopping the Spread of Red Leaf Viruses
• 71: New Techniques to Detect Grapevine Leafroll Disease
• Agri-analysis LLC
• Donate: Juan Nevarez Memorial Scholarship
• Grape Program at Foundation Plant Services
• Leafroll 3 Virus (GLRaV3) AKA Grapevine Leafroll Disease in Washington
• Next Generation Sequencing (Deep Sequencing)
• PCR (Polymerase Chain Reaction) Testing
• Red Blotch Virus
• SIP Certified
• Sustainable Ag Expo November 14-16, 2022

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Craig Macmillan 0:00

My guest today is Alan Wei, who's owner and lab manager of Agri Analysis LLC in Davis, California. Alan, thanks for being on the show.

Alan Wei 0:10

Thank you very much, Craig, for hosting me. And I'm very delighted to be here. And I want to use this opportunity to say hello to listeners as well.

Craig Macmillan 0:19

So Alan, I want to have you on the show, because I want to talk about anything that's new and exciting in the world of grapevine virology, and a lot of research and a lot of development in industry with labs like your own. So, what's what's what's happening out there, what's going on with detection of viruses these days?

Alan Wei 0:36

There is a International conference on grapevine viruses that's held every three years. So last time was 2018, in Chile. And the second, the following time was supposed to be 2021 in Greece, and unfortunately, that was canceled due to the obvious reasons, and then was supposed to be happening this