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Phlebotomy Exam Prep 43, Centrifugation Principles and Time
Published 1 month, 2 weeks ago
Description
This podcast is made by Ran Chen, who holds an EA license, Insurance and Securities licenses (Series 6, 63, 65), and the CFP® designation. He is passionate about opening access to high-quality exam preparation resources and helping learners prepare more effectively for professional certification exams.
In this episode you will learn:
- The standard centrifugation setting for serum and plasma is 10-15 minutes at a Relative Centrifugal Force (RCF) of 1000 to 1300g.
- Why Serum Separator Tubes (SSTs) must be allowed to clot for a full 30 minutes at room temperature before being placed in the centrifuge.
- The critical importance of precisely counterbalancing the centrifuge to ensure sample integrity, prevent mechanical damage, and avoid creating biohazardous aerosols.
- The absolute rule against re-spinning gel separator tubes and the associated risk of falsely elevated potassium levels.
- How using the centrifuge's hard brake can disrupt cellular separation and lead to hemolysis, and why letting it coast to a stop is the correct procedure.
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