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Phlebotomy Exam Prep 11, EDTA (Lavender) Tube — Uses and Mechanism
Published 3 weeks ago
Description
This podcast is made by Ran Chen, who holds an EA license, Insurance and Securities licenses (Series 6, 63, 65), and the CFP® designation. He is passionate about opening access to high-quality exam preparation resources and helping learners prepare more effectively for professional certification exams.
In this episode you will learn:
- EDTA's mechanism of action is chelating calcium ions, which is an essential step in the blood clotting cascade.
- The lavender-top tube is crucial for hematology tests like the CBC and ESR because EDTA preserves the natural size and shape of blood cells.
- Immediately after drawing, the tube must be inverted 8-10 times to thoroughly mix the blood and anticoagulant, preventing microclots.
- A common exam trap is underfilling the tube, which increases the EDTA-to-blood ratio and causes red blood cell shrinkage, leading to inaccurate results like a falsely low hematocrit.
- K2 EDTA is generally preferred over K3 EDTA for hematology studies as it has a lesser impact on red blood cell volume.
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