Episode Details

In this episode, we add another article to our Rapid Fire Journal Club. Luke Hedrick and Dave Furfaro discuss the MIST 2 trial published in NEJM in 2011 evaluating enzymatic therapy for complex parapneumonic effusions and empyemas.

Article and Reference

We are talking today about the MIST 2 trial evaluating the use of intrapleural tPa and DNase for intrapleural infections.

Rahman NM, Maskell NA, West A, Teoh R, Arnold A, Mackinlay C, Peckham D, Davies CW, Ali N, Kinnear W, Bentley A, Kahan BC, Wrightson JM, Davies HE, Hooper CE, Lee YC, Hedley EL, Crosthwaite N, Choo L, Helm EJ, Gleeson FV, Nunn AJ, Davies RJ. Intrapleural use of tissue plasminogen activator and DNase in pleural infection. N Engl J Med. 2011 Aug 11;365(6):518-26. doi: 10.1056/NEJMoa1012740. PMID: 21830966.

Key Learning Points

• • Background:
    • Infections in the pleural space are common and morbid, often requiring surgical intervention. Unfortunately, antibiotics and chest tube drainage often fail. The MIST1 trial (NEJM, 2005) of intrapleural streptokinase showed no benefit. MIST2 studied intrapleural tPA and DNase to ease drainage by breaking down septations and thinning pleural fluid.
  • Study Design (design, primary outcome, participants, etc)
    • Design:
      • Double-blind, double-dummy, 2×2 factorial RCT at 11 UK hospitals from 12/2005 to 11/2008
        • By double dummy, we mean that there was a sham placebo for each of the study drugs
    • Primary Outcome
      • Change in the percent of the hemithorax taken up by effusion on CXR at day 7 compared to day 1
      • Key secondary outcomes:
        • Referral for surgery
        • Hospital LOS
        • All cause 3 month and 12 month mortality
        • AEs
    • Participants
      • Inclusion:
        • Clinical evidence of infection (assessed by recruiting MD; EG, fever, CRP, WBC) and
        • Pleural fluid with any of:
          • Grossly purulent
          • Positive pleural fluid culture or gram stain
          • pH < 7.2
      • Exclusion: aiming to exclude patients with increased bleeding risk or who can’t re-expand the lung after drainage
        • Age < 18
        • Previous intrapleural fibrinolytics, DNase, or both for empyema
        • Allergy to tPA or DNase
        • Coincidental stroke (hemorrhage risk)
        • Major hemorrhage or trauma
        • Major surgery in the last 5 days
        • Previous pneumonectomy on the infected side
        • Pregnancy, lactation
        • Expected survival < 3 months from something other than what caused the pleural problem
      • Summary: Middle-aged, mostly male patients with complicated pleural effusion or empyema occupying 1/3 to 2/5 hemithorax with mostly small-bore CDs for mostly co